pe-labeled anti-cd25 mab rea945  (Miltenyi Biotec)

Journal: Current Issues in Molecular Biology

Article Title: The Enhancement of Regulatory T Cell Maturation and Th1/Th2 Balance through FOXP3 Expression by Lactobacillus paracasei in an Ovalbumin-Induced Allergic Skin Animal Model

doi: 10.3390/cimb46100636

Figure Lengend Snippet: Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) CD4+/CD25+/FOXP3+ in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.

Article Snippet: Cell staining was performed using a panel of monoclonal antibodies: FITC-labeled anti-mouse CD4 (clone: RM4-5, eBioscience, San Diego, CA, USA); PE-labeled rat anti-mouse CD25 (clone: PC61.5, eBioscience); PerCP-Cy™ 5.5-labeled rat anti-mouse IFN-γ (clone: XMG1.2, BD, NJ, USA); PerCP-Cy™-labeled rat anti-mouse IL-4 (clone: 11B11, BioLegend, San Diego, CA, USA); PerCP-Cy™ 5.5-labeled rat anti-mouse FOXP3 (clone: FJK-16s, Invitrogen, Carlsbad, CA, USA); and PerCP-Cy™ 5.5-labeled rat anti-mouse/rat IL-17A (clone: eBio17B7, eBioscience).

Techniques: Comparison, Control, Saline

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: Comparison of possible risk factors and clinical features between Cervical Cancer and Control Participants

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques: Comparison, Control

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: Dot Plot demonstrating 3.02% of non-specific binding (NSB) cells as a proportion of CD4 + CD25 + cells (Upper Right quadrant, Panel C) in a cervical cancer participant. Panel A : Dot plot representation of PBMC according to size (x-axis = forward scatter) and granularity (y-axis = side scatter); the gated region corresponds to lymphocytes. Panel B : Dot plot showing gated lymphocytes from panel A, stained with CD4 (x-axis) and CD25 (y-axis). Panel C : Dot plot showing gated double CD4 and CD25 positive cells (upper right quadrant in panel B) further stained with Isotype control (NSB/dead cells). The upper right quadrant represents cells (positive for CD4, CD25 and NSB)

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques: Binding Assay, Staining, Control

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: Dot Plot demonstrating FoxP3 in the same cervical cancer participant shown in Fig. . 47.33% positivity of FoxP3 as a proportion of CD4 + CD25 + cells (upper right quadrant, Panel C). Panel A : Dot plot representation of PBMC according to size (x-axis = forward scatter) and granularity (y-axis = side scatter); the gated region corresponds to lymphocytes. Panel B : Dot plot showing gated lymphocytes from panel A, stained with CD4 (x-axis) and CD25 (y-axis). Panel C : Dot plot showing gated double CD4 and CD25 positive cells (upper right quadrant in panel B) further stained with FoxP3. The upper right quadrant represents Treg cells (positive for CD4, CD25 and FoxP3).

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques: Staining

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: The bar graph demonstrates the frequency of T regulatory cells as a frequency of CD4 + CD25 + FoxP3 T cells according to HPV types in cervical cancer patients and controls women. No significant difference in Tregs frequency in controls for HPV infections (Kruskal-Wallis (p = 0.7788). Tregs frequency was significantly high in HR HPV cervical cancer compared to controls using the Mann-Whitney test, p < 0.0001). Bars represent the median, and error bars represent the interquartile range (IQR).

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques: MANN-WHITNEY

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: Frequency of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in relation to CD4 count in HIV-infected Participants

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques:

Journal: BMC Cancer

Article Title: Regulatory T cell frequency in peripheral blood of women with advanced cervical Cancer including women living with HIV

doi: 10.1186/s12885-023-11345-9

Figure Lengend Snippet: Univariate logistic regression analysis of Tregs and relevant variables in cervical cancer and Control participants

Article Snippet: The reagents included, APC- labelled FoxP3 (Clone PCH 101), FITC- labelled CD4, PE-labelled CD25, in a phosphate buffer, ph 7.2, 150 Mm NaCl, 0.09% NaN 3, 0.2% BSA, Clone PCH101, Isotype: Rat IgG2a, (Cat.No.88-8998), (eBioscience, San Diego, CA,USA).

Techniques: Control